| Titre : | ASSOCIATION STUDY OF TLR4 RS4986790 POLYMORPHISM WITH COVID-19 DISEASE SEVERITY IN THE MOROCCAN POPULATION | | Type de document : | thèse | | Auteurs : | BOUGARN HALA, Auteur | | Année de publication : | 2024 | | Langues : | Anglais (eng) | | Mots-clés : | TLR4 rs4986790 polymorphism (A>G) COVID-19 Severe and Non-Severe cases Moroccan population Genetic susceptibility Polymorphisme TLR4 rs4986790 (A>G) COVID-19 sévères non-sévères Population marocaine Susceptibilité génétique تعدد الأشكال TLR4 rs4986790 النتائج الحادة لكوفيد- 19 السكان المغاربة الحساسية
الوراثية | | Résumé : | The Toll-like receptor 4 (TLR4) plays a pivotal role in the innate immune response by recognizing pathogen-associated molecular patterns (PAMPs) and Damage associated molecular patterns (DAMPs), including viral components. This retrospective study investigates the impact of the TLR4 rs4986790 polymorphism on susceptibility to COVID-19 severity.
Sixty-three COVID-19 patients were diagnosed using real-time reverse transcription polymerase chain reaction (RT-qPCR) tests on nasopharyngeal swabs, from July 2021 to December 2023 at the Moulay Youssef Hospital and the Ibn Rochd University Hospital (CHU) Casablanca. The patients were clinically divided into two groups: non-severe (n=31) and severe (n=32). The TLR4 rs4986790 (A>G) polymorphism was genotyped using the TaqMan real-time allelic discrimination assay, on blood samples.
Age was identified as a significant risk factor for severe COVID-19 outcomes (p < 0.05). Immunological analysis showed significantly higher levels of anti-RBD IgG in severe COVID-19 patients compared to non-severe cases (p = 0.0011), whereas anti-N IgG index levels did not differ significantly between the two groups (p = 0.1789).
Results revealed genotype distributions (AA, AG, GG) consistent with Hardy-Weinberg equilibrium in both groups (p > 0.05). The frequencies of AA, AG, and GG genotypes were 87.1%, 9.7%, and 3.2% in severe COVID-19 patients, and 90.6%, 9.4%, and 0% in non-severe patients, respectively. Allele frequencies were similar between the severe and non-severe groups (A allele: 55%, G allele: 45%).
Our preliminary data showed that the TLR4 rs4986790 SNP did not affect COVID-19 severity. However, the TLR4 rs4986790 variant may modulate the humoral response against SARS-CoV-2 infection. | | Numéro (Thèse ou Mémoire) : | MM0172024 | | Président : | ANNIZ Tarik | | Directeur : | EZZIKOURI Sayeh | | Juge : | OUADGHIRI Mouna | | Juge : | BENTAYEBI Kaoutar |
ASSOCIATION STUDY OF TLR4 RS4986790 POLYMORPHISM WITH COVID-19 DISEASE SEVERITY IN THE MOROCCAN POPULATION [thèse] / BOUGARN HALA, Auteur . - 2024. Langues : Anglais ( eng) | Mots-clés : | TLR4 rs4986790 polymorphism (A>G) COVID-19 Severe and Non-Severe cases Moroccan population Genetic susceptibility Polymorphisme TLR4 rs4986790 (A>G) COVID-19 sévères non-sévères Population marocaine Susceptibilité génétique تعدد الأشكال TLR4 rs4986790 النتائج الحادة لكوفيد- 19 السكان المغاربة الحساسية
الوراثية | | Résumé : | The Toll-like receptor 4 (TLR4) plays a pivotal role in the innate immune response by recognizing pathogen-associated molecular patterns (PAMPs) and Damage associated molecular patterns (DAMPs), including viral components. This retrospective study investigates the impact of the TLR4 rs4986790 polymorphism on susceptibility to COVID-19 severity.
Sixty-three COVID-19 patients were diagnosed using real-time reverse transcription polymerase chain reaction (RT-qPCR) tests on nasopharyngeal swabs, from July 2021 to December 2023 at the Moulay Youssef Hospital and the Ibn Rochd University Hospital (CHU) Casablanca. The patients were clinically divided into two groups: non-severe (n=31) and severe (n=32). The TLR4 rs4986790 (A>G) polymorphism was genotyped using the TaqMan real-time allelic discrimination assay, on blood samples.
Age was identified as a significant risk factor for severe COVID-19 outcomes (p < 0.05). Immunological analysis showed significantly higher levels of anti-RBD IgG in severe COVID-19 patients compared to non-severe cases (p = 0.0011), whereas anti-N IgG index levels did not differ significantly between the two groups (p = 0.1789).
Results revealed genotype distributions (AA, AG, GG) consistent with Hardy-Weinberg equilibrium in both groups (p > 0.05). The frequencies of AA, AG, and GG genotypes were 87.1%, 9.7%, and 3.2% in severe COVID-19 patients, and 90.6%, 9.4%, and 0% in non-severe patients, respectively. Allele frequencies were similar between the severe and non-severe groups (A allele: 55%, G allele: 45%).
Our preliminary data showed that the TLR4 rs4986790 SNP did not affect COVID-19 severity. However, the TLR4 rs4986790 variant may modulate the humoral response against SARS-CoV-2 infection. | | Numéro (Thèse ou Mémoire) : | MM0172024 | | Président : | ANNIZ Tarik | | Directeur : | EZZIKOURI Sayeh | | Juge : | OUADGHIRI Mouna | | Juge : | BENTAYEBI Kaoutar |
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