| Titre : | EXPLORING MICROBIAL DIVERSITY OF SEBOU’S RIVER USING A METAGENOMIC APPROACH | | Type de document : | thèse | | Auteurs : | Ikram GARBOUNI, Auteur | | Année de publication : | 2020 | | Langues : | Anglais (eng) | | Mots-clés : | Sebou river Metagenomics Pollution Taxonomy profiling Biodiversity Microbiome La rivière de Sebou Métagénomique Profil taxonomique Biodiversité Microbiome | | Résumé : | Sebou river is the second largest river in Morocco, starting from a source in the middle Atlas
at an altitude of 2800m, traversing 600Km, and draining 40,000Km 2 , and flows into the
Atlantic Ocean near the city of Kenitra . This study aims to explore the microbial
communities in the Sebou river and its variation between two seasons, Summer and Winter,
and during three consecutive years. This will help to determine the impact of pollution on
Sebou’s microbial communities, and defining any sources of pathogenic infection might
come from this river. In order to identify Operational Taxonomy Units (OTUs) from the 6
samples, the Metagenomics Micca package was used. Phyloseq bioinformatic tool has been
used to determine the alpha and beta diversities of the river samples, as well as the taxonomy
profile of each sample. Results showed that alpha diversity decreased from 2014 to 2016 in
both summer and winter. Indeed, Shanon Index for summer went from 9 (2014) to 8 (2015),
to 6.8 (2016)) while for winter, it went from 8 (2014) to 6.7 (2015), to 3 (2016). The alpha
diversity of summer’s communities was always higher than winter’s one. BOD5, COD, and
NO 3- increased from 2014 to 2016, indicating that the diversity most probably decreased due
to the high pollution levels in the river sampling areas. The taxonomic profiles of Sebou’s
river include 36 families and 58 Genera. Taxonomy profiles showed the dominance of the
Moraxellaceae family in 2016, especially the genus Acinetobacter, these are known as
pathogenic agents that might cause pneumonia, bloodstream infection, and urinary tract
infections. Such findings may contribute to characterizing Moroccan the microbial river
inventories. | | Numéro (Thèse ou Mémoire) : | MM0362020 | | Président : | IBRAHIMI Azeddine | | Directeur : | GHAZAL Hassan | | Juge : | ALLALI Imane | | Juge : | HAMMANI Khalil | | Juge : | CHAHBOUNE Rajae |
EXPLORING MICROBIAL DIVERSITY OF SEBOU’S RIVER USING A METAGENOMIC APPROACH [thèse] / Ikram GARBOUNI, Auteur . - 2020. Langues : Anglais ( eng) | Mots-clés : | Sebou river Metagenomics Pollution Taxonomy profiling Biodiversity Microbiome La rivière de Sebou Métagénomique Profil taxonomique Biodiversité Microbiome | | Résumé : | Sebou river is the second largest river in Morocco, starting from a source in the middle Atlas
at an altitude of 2800m, traversing 600Km, and draining 40,000Km 2 , and flows into the
Atlantic Ocean near the city of Kenitra . This study aims to explore the microbial
communities in the Sebou river and its variation between two seasons, Summer and Winter,
and during three consecutive years. This will help to determine the impact of pollution on
Sebou’s microbial communities, and defining any sources of pathogenic infection might
come from this river. In order to identify Operational Taxonomy Units (OTUs) from the 6
samples, the Metagenomics Micca package was used. Phyloseq bioinformatic tool has been
used to determine the alpha and beta diversities of the river samples, as well as the taxonomy
profile of each sample. Results showed that alpha diversity decreased from 2014 to 2016 in
both summer and winter. Indeed, Shanon Index for summer went from 9 (2014) to 8 (2015),
to 6.8 (2016)) while for winter, it went from 8 (2014) to 6.7 (2015), to 3 (2016). The alpha
diversity of summer’s communities was always higher than winter’s one. BOD5, COD, and
NO 3- increased from 2014 to 2016, indicating that the diversity most probably decreased due
to the high pollution levels in the river sampling areas. The taxonomic profiles of Sebou’s
river include 36 families and 58 Genera. Taxonomy profiles showed the dominance of the
Moraxellaceae family in 2016, especially the genus Acinetobacter, these are known as
pathogenic agents that might cause pneumonia, bloodstream infection, and urinary tract
infections. Such findings may contribute to characterizing Moroccan the microbial river
inventories. | | Numéro (Thèse ou Mémoire) : | MM0362020 | | Président : | IBRAHIMI Azeddine | | Directeur : | GHAZAL Hassan | | Juge : | ALLALI Imane | | Juge : | HAMMANI Khalil | | Juge : | CHAHBOUNE Rajae |
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