| Titre : | COMPARATIVE GENOMIC ANALYSIS OF FOUR MOROCCAN CLINICAL STRAINS OF KLEBSIELLA PNEUMONIAE DISPLAYING DIFFERENT ANTIBIOTIC RESISTANCE PROFILES | | Type de document : | thèse | | Auteurs : | Zineb KABBAJ, Auteur | | Année de publication : | 2020 | | Langues : | Anglais (eng) | | Mots-clés : | Klebsiella pneumoniae outbreak comparative genomic antimicrobial resistance MLST Plasmids Pan-genome Klebsiella pneumoniae épidémie génomique comparative résistance aux
antimicrobiens MLST Plasmides Pan-génome | | Résumé : | Klebsiella Pneumoniae is now recognized as a major threat to human health globally because
of the emergence of multidrug-resistant strains associated with hospital outbreaks and
hypervirulent strains associated with severe community-acquired infections. However, very
little research work has been carried out concerning the sequencing of the complete genome of
K. pneumoniae.
The objective of this study is to carry out a genomic analysis of four strains of K. Pneumoniae
with different AMR profiles from different Moroccan patients in order to identify the resistance
genes and perform a genomic comparison.
Four strains of K. Pneumoniae were subjects of a de Novo assembly followed by an annotation
in order to identify the genetic elements (resistance genes, plasmids...) of the strains. This
genomic analysis pipeline made it possible to identify 31 resistance genes from different
antimicrobial classes (β-lactam, aminoglycosides, Fosfomycin, fluoroquinolone…), 8
plasmids of different types (IncF, IncX, IncR, IncC, Col), 4 sequence types using MLST
(ST664, ST13, ST2695, ST35) and a pan-genome of 4299 core genes and 1946 unique genes.
Our study provided a global view of the information carried in the genome of K. Pneumoniae.
There are multiple resistance genes across diverse Kp genomes and plasmids in circulation that
are capable of carrying this resistance. Unless appropriate interventions are rapidly placed,
these may lead to a massive problem of untreatable infection in vulnerable populations. | | Numéro (Thèse ou Mémoire) : | MM0492020 | | Président : | Azeddine IBRAHIMI | | Directeur : | Tarek ALOUANE ; Latefa BISKRI | | Juge : | Mouna OUADGHIRI | | Juge : | Souad KARTTI |
COMPARATIVE GENOMIC ANALYSIS OF FOUR MOROCCAN CLINICAL STRAINS OF KLEBSIELLA PNEUMONIAE DISPLAYING DIFFERENT ANTIBIOTIC RESISTANCE PROFILES [thèse] / Zineb KABBAJ, Auteur . - 2020. Langues : Anglais ( eng) | Mots-clés : | Klebsiella pneumoniae outbreak comparative genomic antimicrobial resistance MLST Plasmids Pan-genome Klebsiella pneumoniae épidémie génomique comparative résistance aux
antimicrobiens MLST Plasmides Pan-génome | | Résumé : | Klebsiella Pneumoniae is now recognized as a major threat to human health globally because
of the emergence of multidrug-resistant strains associated with hospital outbreaks and
hypervirulent strains associated with severe community-acquired infections. However, very
little research work has been carried out concerning the sequencing of the complete genome of
K. pneumoniae.
The objective of this study is to carry out a genomic analysis of four strains of K. Pneumoniae
with different AMR profiles from different Moroccan patients in order to identify the resistance
genes and perform a genomic comparison.
Four strains of K. Pneumoniae were subjects of a de Novo assembly followed by an annotation
in order to identify the genetic elements (resistance genes, plasmids...) of the strains. This
genomic analysis pipeline made it possible to identify 31 resistance genes from different
antimicrobial classes (β-lactam, aminoglycosides, Fosfomycin, fluoroquinolone…), 8
plasmids of different types (IncF, IncX, IncR, IncC, Col), 4 sequence types using MLST
(ST664, ST13, ST2695, ST35) and a pan-genome of 4299 core genes and 1946 unique genes.
Our study provided a global view of the information carried in the genome of K. Pneumoniae.
There are multiple resistance genes across diverse Kp genomes and plasmids in circulation that
are capable of carrying this resistance. Unless appropriate interventions are rapidly placed,
these may lead to a massive problem of untreatable infection in vulnerable populations. | | Numéro (Thèse ou Mémoire) : | MM0492020 | | Président : | Azeddine IBRAHIMI | | Directeur : | Tarek ALOUANE ; Latefa BISKRI | | Juge : | Mouna OUADGHIRI | | Juge : | Souad KARTTI |
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